Expanding plant genome-editing scope by an engineered iSpyMacCas9 system that targets A-rich PAM sequences

Sretenovic, S; Yin, DS; Levav, A; Selengut, JD; Mount, SM; Qi, YP

Qi, YP (corresponding author), Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA.; Qi, YP (corresponding author), Univ Maryland, Inst Biosci & Biotechnol Res, Rockville, MD 20850 USA.

PLANT COMMUNICATIONS, 2021; 2 (2):

Abstract

The most popular CRISPR-SpCas9 system recognizes canonical NGG protospacer adjacent motifs (PAMs). Previously engineered SpCas9 variants, such as Cas9......

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