Scalable Transfection of Maize Mesophyll Protoplasts

Tonnies, J; Mueth, NA; Gorjifard, S; Chu, J; Queitsch, C

Mueth, NA; Queitsch, C (通讯作者),Univ Washington, Genome Sci Dept, Seattle, WA 98195 USA.

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2023; (196):

Abstract

The transfection of maize mesophyll cells often involves digesting the plant cell walls to create protoplasts and then inserting DNA via electroporati......

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