Mutagenesis:使用彗星测定的方法来研究眼部细胞DNA损伤!

2017-11-18 cuiguizhong MedSci原创

西班牙纳瓦拉大学药学与科学学院食品科学与毒理学系的Azqueta A近日在Mutagenesis杂志上发表了他们近期的一项工作,他们介绍了使用彗星测定的方法来研究眼部细胞DNA损伤的情况。

西班牙纳瓦拉大学药学与科学学院食品科学与毒理学系的Azqueta A近日在Mutagenesis杂志上发表了他们近期的一项工作,他们介绍了使用彗星测定的方法来研究眼部细胞DNA损伤的情况。

目前,对于人眼来源的细胞进行DNA损伤的研究还很少。已经有一些临床研究,他们使用来自手术切除的组织细胞,使用彗星测定的方法来确定DNA突变的碱基以及DNA链断裂的情况。用于研究的组织包括角膜上皮和内皮、晶状体囊、虹膜和视网膜色素上皮等。

眼睛作为视觉器官,为了其生物监测目的,常暴露于有潜在诱变剂存在的环境中。与皮肤相比,眼睛没有保护作用,这也是研究意义所在。使用非侵入性的技术手段,从眼泪中收集泪液管细胞,或通过细胞学的方法从眼表获取细胞进行科学研究将是必要的。然而,作者也指出,这些方法需要进一步的发展和验证。

原文出处:

Azqueta, A., et al. The comet assay applied to cells of the eye. Mutagenesis, 2017.

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    2018-06-15 huiwelcome
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    2018-03-20 cy0324
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    2017-11-20 skhzy
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    2017-11-18 天涯183

    非常好的文章.学习了

    0