Neural Regen Res.：脊柱图像引导放疗有助于保护脊髓神经元

《中国神经再生研究（英文版）》杂志于2012年12月35期出版的一项关于“Spinal cord biological safety of image-guided radiation therapy versus conventional radiation therapy”的研究，以犬T9-10椎体模拟肿瘤侵袭椎体，通过对脊髓组织病理学、超微结构、神经元凋亡及凋亡相关蛋白Fas和热休克蛋白70表达的检测发现，脊柱图像引导放疗以对脊髓的可逆性损伤为主，而普通放疗以凋亡为主。在相同放射治疗剂量下，与普通放疗比较，脊柱图像引导放疗引起的细胞凋亡数明显降低，凋亡促进蛋白Fas的表达量明显减少，而凋亡抑制蛋白热休克蛋白70表达量明显增多；同时脊髓细胞凋亡指数与Fas/热休克蛋白70呈正相关。作者认为，脊柱图像引导放疗对脊髓神经元的生物安全性优于普通放疗。

doi:10.3969/j.issn.1673-5374.2012.35.002
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Spinal cord biological safety of image-guided radiation therapy versus conventional radiation therapy

Wanlong Xu1, 2, Xilinbaoleri1, Hao Liu1, Ruozheng Wang1, Jingping Bai1

Tumor models were simulated in purebred Beagles at the T9-10 levels of the spinal cord and treated with spinal image-guided radiation therapy or conventional radiation therapy with 50 or 70 Gy total radiation. Three months after radiation, neuronal injury at the T9-10 levels was observed, including reversible injury induced by spinal image-guided radiation therapy and apoptosis induced by conventional radiation therapy. The number of apoptotic cells and expression of the proapoptotic protein Fas were significantly reduced, but expression of the anti-apoptotic protein heat shock protein 70 was significantly increased after image-guided radiation therapy compared with the conventional method of the same radiation dose. Moreover, the spinal cord cell apoptotic index positively correlated with the ratio of Fas/heat shock protein 70. These findings indicate that 3 months of radiation therapy can induce a late response in the spinal cord to radiation therapy; image-guided radiation therapy is safer and results in less neuronal injury compared with conventional radiation therapy.