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ACS Appl Mater Interfaces：Ca/siRNA钛表面涂层可有效控制间充质干细胞的分化

2018-03-16 MedSci MedSci原创

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通过RNA干扰（RNAi）控制间充质干细胞（MSC）分化是下一代再生医学的一种有希望的方法。然而，RNAi的高效递送仍然是一个限制因素。在这项研究中，我们开发了一种简单的，生物相容的，高效的小分子RNA治疗方法，通过钙离子从种植体表面进入人类MSCs（hMSCs）。 首先，我们证明简单Ca/siRNA靶向绿色荧光蛋白（GFP）纳米复合物能够在具有足够Ca2+浓度（> 5mM）的表达GF

通过RNA干扰（RNAi）控制间充质干细胞（MSC）分化是下一代再生医学的一种有希望的方法。然而，RNAi的高效递送仍然是一个限制因素。在这项研究中，我们开发了一种简单的，生物相容的，高效的小分子RNA治疗方法，通过钙离子从种植体表面进入人类MSCs（hMSCs）。

首先，我们证明简单Ca/siRNA靶向绿色荧光蛋白（GFP）纳米复合物能够在具有足够Ca2+浓度（> 5mM）的表达GFP的hMSCs中有效沉默GFP。此外，单次转染可获得超过2周的持久沉默效应。所有三种主要的胞吞作用途径（网格蛋白和小窝蛋白介导的内吞作用和巨胞吞作用）参与了MSCs对Ca/siRNA复合物的内化，并且巨胞吞作用占主导地位。此外，当用阳极氧化预处理以产生纳米管（NT）层时，Ca/siRNA复合物可以被有效地加载到钛植体表面。

由于NT表面的亲水性，Ca/siRNA以高效率（接近100％）快速加载（小于4小时），形成均匀的无定形涂层。Ca/siRNA包被的NT表面在2小时内显示出80％的siRNA复合物的初始突释，足以实现对接触的hMSCs的稳健基因沉默。为了证明我们的Ca/siRNA包被技术的治疗潜力，将Ca/antimiR-138复合物加载到NT表面，可明显增强hMSCs的成骨分化。

总之，我们的研究结果表明，Ca2+是一种有效的生物相容性载体，可将小RNA治疗药物输送到hMSCs中，包括溶液和功能化表面，这为控制MSC分化和组织再生提供了一种新方法。

原始出处：

Song W, Yang C, et al., Calcium-MicroRNA Complex-Functionalized Nanotubular Implant Surface for Highly Efficient Transfection and Enhanced Osteogenesis of Mesenchymal Stem Cells. ACS Appl Mater Interfaces. 2018 Mar 7;10(9):7756-7764. doi: 10.1021/acsami.7b18289. Epub 2018 Feb 22.

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    2018-04-09 30397613

    #钛#

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    2018-08-10 夏天与十三菇凉

    #FAC#

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    2018-06-03 h.y200607_23674418

    #间充质干细#

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=1679690, encodeId=5f9f16e9690d3, content=<a href='/topic/show?id=d4ac9639efa' target=_blank style='color:#2F92EE;'>#钛#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=96397, encryptionId=d4ac9639efa, topicName=钛)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=927227807332, createdName=30397613, createdTime=Mon Apr 09 15:02:00 CST 2018, time=2018-04-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1956678, encodeId=306919566e8e7, content=<a href='/topic/show?id=7647e210ac' target=_blank style='color:#2F92EE;'>#FAC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=7210, encryptionId=7647e210ac, topicName=FAC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdqq.qlogo.cn/qqapp/1103841572/B389FA68ECBF51BE2FE14093CF61F6AE/100, createdBy=eb8e2500096, createdName=夏天与十三菇凉, createdTime=Fri Aug 10 02:02:00 CST 2018, time=2018-08-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1815307, encodeId=592f181530e79, content=<a href='/topic/show?id=ae359e231d4' target=_blank style='color:#2F92EE;'>#间充质干细#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=97231, encryptionId=ae359e231d4, topicName=间充质干细)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4b632500090, createdName=h.y200607_23674418, createdTime=Sun Jun 03 09:02:00 CST 2018, time=2018-06-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1655841, encodeId=c82116558412a, content=<a href='/topic/show?id=2a011829d7' target=_blank style='color:#2F92EE;'>#ACE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=1829, encryptionId=2a011829d7, topicName=ACE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=635024767990, createdName=shanyongle, createdTime=Fri Oct 26 07:02:00 CST 2018, time=2018-10-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=297647, encodeId=662929e647f8, content=一起学习学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/TDiaAWyicHgedzX3wKo3qcxudVJib7AfibfvNeufHRF2jG3Bw8MmZyWjIhtBV1mpJI7Sy9n7RcBKK5t3CuOKuOFxkg/0, createdBy=dd702206933, createdName=wzb521zf, createdTime=Mon Mar 19 07:26:10 CST 2018, time=2018-03-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1279240, encodeId=36ad12e92405b, content=<a href='/topic/show?id=316d1882bc' target=_blank style='color:#2F92EE;'>#ACS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=1882, encryptionId=316d1882bc, topicName=ACS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=102a153, createdName=yaanren, createdTime=Sun Mar 18 12:02:00 CST 2018, time=2018-03-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1548196, encodeId=9c3a1548196e0, content=<a href='/topic/show?id=c87e16332da' target=_blank style='color:#2F92EE;'>#siRNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=16332, encryptionId=c87e16332da, topicName=siRNA)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=271114029857, createdName=12498ebem23暂无昵称, createdTime=Sun Mar 18 12:02:00 CST 2018, time=2018-03-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=296971, encodeId=57702969e1b8, content=学习一下谢谢, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mONcle9pic3zSn0GqHrsfLGSUSR8zwnWWLT4FMl5XH8S58HuZslVkLsAPbXDXaliagK7TOxQfmngKibG1yrDonepCLDkcxGk8hS/0, createdBy=b8282062892, createdName=changjiu, createdTime=Sat Mar 17 05:04:50 CST 2018, time=2018-03-17, status=1, ipAttribution=)]
    2018-10-26 shanyongle

    #ACE#

    0

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    2018-03-19 wzb521zf

    一起学习学习

    0

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    2018-03-18 yaanren

    #ACS#

    0

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    2018-03-18 12498ebem23暂无昵称

    #siRNA#

    0

  8. [GetPortalCommentsPageByObjectIdResponse(id=1679690, encodeId=5f9f16e9690d3, content=<a href='/topic/show?id=d4ac9639efa' target=_blank style='color:#2F92EE;'>#钛#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=96397, encryptionId=d4ac9639efa, topicName=钛)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=927227807332, createdName=30397613, createdTime=Mon Apr 09 15:02:00 CST 2018, time=2018-04-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1956678, encodeId=306919566e8e7, content=<a href='/topic/show?id=7647e210ac' target=_blank style='color:#2F92EE;'>#FAC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=7210, encryptionId=7647e210ac, topicName=FAC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdqq.qlogo.cn/qqapp/1103841572/B389FA68ECBF51BE2FE14093CF61F6AE/100, createdBy=eb8e2500096, createdName=夏天与十三菇凉, createdTime=Fri Aug 10 02:02:00 CST 2018, time=2018-08-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1815307, encodeId=592f181530e79, content=<a href='/topic/show?id=ae359e231d4' target=_blank style='color:#2F92EE;'>#间充质干细#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=97231, encryptionId=ae359e231d4, topicName=间充质干细)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4b632500090, createdName=h.y200607_23674418, createdTime=Sun Jun 03 09:02:00 CST 2018, time=2018-06-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1655841, encodeId=c82116558412a, content=<a href='/topic/show?id=2a011829d7' target=_blank style='color:#2F92EE;'>#ACE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=1829, encryptionId=2a011829d7, topicName=ACE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=635024767990, createdName=shanyongle, createdTime=Fri Oct 26 07:02:00 CST 2018, time=2018-10-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=297647, encodeId=662929e647f8, content=一起学习学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/TDiaAWyicHgedzX3wKo3qcxudVJib7AfibfvNeufHRF2jG3Bw8MmZyWjIhtBV1mpJI7Sy9n7RcBKK5t3CuOKuOFxkg/0, createdBy=dd702206933, createdName=wzb521zf, createdTime=Mon Mar 19 07:26:10 CST 2018, time=2018-03-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1279240, encodeId=36ad12e92405b, content=<a href='/topic/show?id=316d1882bc' target=_blank style='color:#2F92EE;'>#ACS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=1882, encryptionId=316d1882bc, topicName=ACS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=102a153, createdName=yaanren, createdTime=Sun Mar 18 12:02:00 CST 2018, time=2018-03-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1548196, encodeId=9c3a1548196e0, content=<a href='/topic/show?id=c87e16332da' target=_blank style='color:#2F92EE;'>#siRNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=16332, encryptionId=c87e16332da, topicName=siRNA)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=271114029857, createdName=12498ebem23暂无昵称, createdTime=Sun Mar 18 12:02:00 CST 2018, time=2018-03-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=296971, encodeId=57702969e1b8, content=学习一下谢谢, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mONcle9pic3zSn0GqHrsfLGSUSR8zwnWWLT4FMl5XH8S58HuZslVkLsAPbXDXaliagK7TOxQfmngKibG1yrDonepCLDkcxGk8hS/0, createdBy=b8282062892, createdName=changjiu, createdTime=Sat Mar 17 05:04:50 CST 2018, time=2018-03-17, status=1, ipAttribution=)]
    2018-03-17 changjiu

    学习一下谢谢

    0