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Nat Commun：研究追踪揭示小鼠胰腺发育的谱系动态

2018-09-28 MedSci MedSci原创

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器官发生需要多个细胞谱系的复杂相互作用，这些谱系随着时间的推移协调其扩增、分化和成熟。在这里，我们使用单细胞RNA测序、免疫荧光、原位杂交和遗传谱系追踪，在发育时间内分析小鼠胰腺的上皮和间充质区室内的细胞类型。结果发现，以前未被充分认识的发育中间质的细胞异质性，并重建胰腺间皮细胞和间充质细胞类型之间潜在的谱系关系。在上皮细胞内，我们发现先前未描述的内分泌祖细胞群，以及人胎儿组织和人胚胎干细胞中分化

器官发生需要多个细胞谱系的复杂相互作用，这些谱系随着时间的推移协调其扩增、分化和成熟。在这里，我们使用单细胞RNA测序、免疫荧光、原位杂交和遗传谱系追踪，在发育时间内分析小鼠胰腺的上皮和间充质区室内的细胞类型。

结果发现，以前未被充分认识的发育中间质的细胞异质性，并重建胰腺间皮细胞和间充质细胞类型之间潜在的谱系关系。在上皮细胞内，我们发现先前未描述的内分泌祖细胞群，以及人胎儿组织和人胚胎干细胞中分化为胰腺β细胞命运的类似群体。此外，我们沿着该群体的分化轨迹向α或β细胞谱系鉴定了候选转录调节因子。

综上所述，该研究结果确立了胰腺发育的路线图，并证明了这种方法在了解发育中器官的谱系动力学方面的广泛用途。

原始出处：

Lauren E. Byrnes, Daniel M. Wong, et al., Lineage dynamics of murine pancreatic development at single-cell resolution. Nat Commun. 2018; 9: 3922.


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#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=2087432, encodeId=83e6208e43276, content=<a href='/topic/show?id=490750196b' target=_blank style='color:#2F92EE;'>#COMMUN#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=17, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5019, encryptionId=490750196b, topicName=COMMUN)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=7931272, createdName=liuli5079, createdTime=Fri Nov 09 09:25:00 CST 2018, time=2018-11-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1883335, encodeId=6d64188333553, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Mon Dec 17 11:25:00 CST 2018, time=2018-12-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1347331, encodeId=8bf0134e331df, content=<a href='/topic/show?id=88713e938c1' target=_blank style='color:#2F92EE;'>#发育#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=17, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=37938, encryptionId=88713e938c1, topicName=发育)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f4c124, createdName=neurowu, createdTime=Sun Sep 30 12:25:00 CST 2018, time=2018-09-30, status=1, ipAttribution=)]
    2018-11-09 liuli5079

    #COMMUN#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=2087432, encodeId=83e6208e43276, content=<a href='/topic/show?id=490750196b' target=_blank style='color:#2F92EE;'>#COMMUN#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=17, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5019, encryptionId=490750196b, topicName=COMMUN)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=7931272, createdName=liuli5079, createdTime=Fri Nov 09 09:25:00 CST 2018, time=2018-11-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1883335, encodeId=6d64188333553, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Mon Dec 17 11:25:00 CST 2018, time=2018-12-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1347331, encodeId=8bf0134e331df, content=<a href='/topic/show?id=88713e938c1' target=_blank style='color:#2F92EE;'>#发育#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=17, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=37938, encryptionId=88713e938c1, topicName=发育)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f4c124, createdName=neurowu, createdTime=Sun Sep 30 12:25:00 CST 2018, time=2018-09-30, status=1, ipAttribution=)]
    2018-12-17 liye789132251

    #Nat#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=2087432, encodeId=83e6208e43276, content=<a href='/topic/show?id=490750196b' target=_blank style='color:#2F92EE;'>#COMMUN#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=17, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5019, encryptionId=490750196b, topicName=COMMUN)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=7931272, createdName=liuli5079, createdTime=Fri Nov 09 09:25:00 CST 2018, time=2018-11-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1883335, encodeId=6d64188333553, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Mon Dec 17 11:25:00 CST 2018, time=2018-12-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1347331, encodeId=8bf0134e331df, content=<a href='/topic/show?id=88713e938c1' target=_blank style='color:#2F92EE;'>#发育#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=17, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=37938, encryptionId=88713e938c1, topicName=发育)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f4c124, createdName=neurowu, createdTime=Sun Sep 30 12:25:00 CST 2018, time=2018-09-30, status=1, ipAttribution=)]
    2018-09-30 neurowu

    #发育#

    0