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Sci Rep：诊断蜱传疾病的多重血清学平台

2018-02-28 MedSci MedSci原创

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蜱传疾病是美国最常见的媒介传播疾病，血清学是诊断的主要方法。我们开发了第一种基于阵列的多重蜱传播疾病血清学诊断方法，称为TBD-Serochip。TBD-Serochip旨在区分美国8种主要蜱传疾病病原体的抗体反应，包括噬粒吞噬体，果式巴贝虫，博氏疏螺旋体，米氏疏螺旋体，查菲埃立克体，立氏立克次体，Heartland病毒和波瓦桑病毒。每个测定包含大约170,000个12-mer线性肽，其沿着来自每

蜱传疾病是美国最常见的媒介传播疾病，血清学是诊断的主要方法。我们开发了第一种基于阵列的多重蜱传播疾病血清学诊断方法，称为TBD-Serochip。

TBD-Serochip旨在区分美国8种主要蜱传疾病病原体的抗体反应，包括噬粒吞噬体，果式巴贝虫，博氏疏螺旋体，米氏疏螺旋体，查菲埃立克体，立氏立克次体，Heartland病毒和波瓦桑病毒。每个测定包含大约170,000个12-mer线性肽，
其沿着来自每种试剂的主要抗原的蛋白质序列铺平，具有11个氨基酸重叠。这可以准确鉴定各种特定的免疫显性IgG和IgM表位，然后可以用它们来提高诊断准确性，并将鉴别诊断整合到单个测定中。

为了测试TBD-Serochip的表现，我们检查了莱姆病，巴贝斯虫病，边虫病和波氏病毒病患者的血清。我们确定了各种特定的识别性表位，有助于准确诊断每种疾病。我们还确定了以前未确诊的感染。

我们的结果表明，对于现有血清学检测不能进行的TBDs鉴别诊断，TBD-Serochip是一个有前景的工具。

原始出处：

Tokarz R, Mishra N, et al., A multiplex serologic platform for diagnosis of tick-borne diseases. Sci Rep. 2018 Feb 16;8(1):3158. doi: 10.1038/s41598-018-21349-2.

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    2018-03-05 yfjms

    学习

    0

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    2018-03-02 jjjiang0201

    #血清学#

    0

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    2018-03-02 nakerunner

    #蜱#

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    2018-03-01 yfjms

    学习

    0