CRISPR-Cas9, CRISPRi and CRISPR-BEST-mediated genetic manipulation in streptomycetes

Tong, Y; Whitford, CM; Blin, K; Jorgensen, TS; Weber, T; Lee, SY

Weber, T; Lee, SY (corresponding author), Tech Univ Denmark, Novo Nordisk Fdn Ctr Biosustainabil, Lyngby, Denmark.; Lee, SY (corresponding author), Korea Adv Inst Sci & Technol KAIST, Inst BioCentury, Ctr Syst & Synthet Biotechnol, Dept Chem & Biomol Engn,BK21 Plus Program,Metab &, Daejeon, South Korea.

NATURE PROTOCOLS, 2020; 15 (8): 2470

Abstract

Streptomycetes are prominent sources of bioactive natural products, but metabolic engineering of the natural products of these organisms is greatly hi......

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