In situ hybridization. In situ hybridization was performed as described previously (43). cRNAs were prepared from linearized cDNA templates of murine RAMP2 (ACACTTTGCGAACTGCTCCCTGGTGCAGCCCACCTTCTCTGATCCCCCAGAGGATGTGCTCCTGGCCA TGATCATAGCCCCCATCTGCCTCATCCCGTTCCTTGTTACTCTTGTGGT GTGGAGGAGTAAAGACAGCGATGCCCAGGCCTAGGGTCCATTTCTCAGCAGCCATTTTTCCCCCCTTTTCCC TGCTGGAACCAGGAATGGCGCTCCTCCCCTCCCTACCCACTTACTC TCATCCTTCCCACAGACCTGTGGATTGGTGGAAATGGCAGC-AAAGGGGACTCACGACACAATG) to generate antisense and sense probes. The cRNA transcripts were synthesized according to the manufacturer’s instructions (Ambion).