ARCH PATHOL LAB MED：将Cervista人乳头瘤病毒16/18检测方法纳入对福尔马林固定、石蜡包埋头颈部鳞癌标本中人乳头瘤病毒状态的分类算法中

2019-05-09 不详网络

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前期研究表明，采用人乳头瘤病毒(HPV) DNA原位杂交(ISH)和p16免疫组化(IHC)检测口咽部鳞状细胞癌(SCC)中福尔马林固定石蜡包埋(FFPE)组织中高危HPV状态。虽然p16 IHC和HPV DNA ISH联合检测HPV可获得较高的敏感性和特异性，但这些检测之间偶尔的差异已促使对口咽鳞癌患者FFPE组织中Cervista HPV检测的评估。

本研究的目的是比较Cervista HPV 16/18和Cervista HPV HR检测与HPV DNA ISH和p16 IHC检测在口咽源性头颈部鳞癌FFPE组织中的效果。

研究人员使用Cervista HPV16/18检测84例口咽源性鳞癌患者存档的FFPE组织和可用的HPV DNA ISH及p16 IHC检测结果，并通过基于聚合酶链反应(PCR)的HPV16/18基因分型检测进一步验证存在差异的病例。

研究发现，在84个样本中，75%为阳性(84个样本中的63个)，16%的p16免疫组化和HPV DNA ISH检测结果有差异或不明显(84个样本中的13个)。使用Cervista HPV检测，无论是为了澄清差异或不明显的结果，还是作为初次p16 IHC/HPV DNA ISH检测后的确认，81%(84例中的68例)诊断结果明确。13例HPV DNA ISH结果不一致或不明确的患者中，有5例经Cervista HPV 16/18确诊为HPV16或HPV18阳性，并经过基于pcr的HPV 16/18基因分型进一步证实。

研究表明，在确定口咽部鳞状细胞癌患者的FFPE组织标本中的HPV状态时，Cervista HPV检测是一种较为合适的HPV DNA ISH替代方法。

原始出处：

Ming Guo, MD; Abha Khanna, MS;Incorporation of Cervista Human Papillomavirus 16/18 Assay Into Algorithms for Classifying Human Papillomavirus Status in Formalin-Fixed, Paraffin-Embedded Head and Neck Squamous Carcinoma Specimens

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2019-05-30 yb6560

#Pathol#

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2019-06-14 muzishouyi

#VISTA#

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2019-12-01 ms306040998833292

#标本#

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style='color:#2F92EE;'>#鳞癌#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=102944, encryptionId=3502102944e7, topicName=鳞癌)], attachment=null, authenticateStatus=null, createdAvatar=Bioon/avatar/20171006/a73877485ccdf6add82293e671bebe21.jpg, createdBy=745a7977375, createdName=1696533704_45270456, createdTime=Sat May 11 03:59:00 CST 2019, time=2019-05-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1491843, encodeId=abd6149184359, content=<a href='/topic/show?id=b4f14442346' target=_blank style='color:#2F92EE;'>#头颈部#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=44423, encryptionId=b4f14442346, topicName=头颈部)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=0b2d8903151, createdName=wshxjq, createdTime=Sat May 11 03:59:00 CST 2019, time=2019-05-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1589800, encodeId=211a15898006a, content=<a href='/topic/show?id=124d10065220' target=_blank style='color:#2F92EE;'>#颈部#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100652, encryptionId=124d10065220, topicName=颈部)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c46a17776631, createdName=木头人519, createdTime=Sat May 11 03:59:00 CST 2019, time=2019-05-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1609764, encodeId=ab121609e6429, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, 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2019-05-11 ms3297076796458174

#人乳头瘤病毒#

46 0
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time=2019-05-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1589800, encodeId=211a15898006a, content=<a href='/topic/show?id=124d10065220' target=_blank style='color:#2F92EE;'>#颈部#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100652, encryptionId=124d10065220, topicName=颈部)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c46a17776631, createdName=木头人519, createdTime=Sat May 11 03:59:00 CST 2019, time=2019-05-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1609764, encodeId=ab121609e6429, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, 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2019-05-11 gracezdd

#检测方法#

32 0
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time=2019-05-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1589800, encodeId=211a15898006a, content=<a href='/topic/show?id=124d10065220' target=_blank style='color:#2F92EE;'>#颈部#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100652, encryptionId=124d10065220, topicName=颈部)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c46a17776631, createdName=木头人519, createdTime=Sat May 11 03:59:00 CST 2019, time=2019-05-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1609764, encodeId=ab121609e6429, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, 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2019-05-11 1696533704_45270456

#鳞癌#

32 0
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time=2019-05-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1589800, encodeId=211a15898006a, content=<a href='/topic/show?id=124d10065220' target=_blank style='color:#2F92EE;'>#颈部#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100652, encryptionId=124d10065220, topicName=颈部)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c46a17776631, createdName=木头人519, createdTime=Sat May 11 03:59:00 CST 2019, time=2019-05-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1609764, encodeId=ab121609e6429, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Sat May 11 03:59:00 CST 2019, time=2019-05-11, status=1, ipAttribution=)]
2019-05-11 wshxjq

#头颈部#

27 0
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2019-05-11 木头人519

#颈部#

28 0
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2019-05-11 ms3994565386320060

#Med#

25 0

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ARCH PATHOL LAB MED：将Cervista人乳头瘤病毒16/18检测方法纳入对福尔马林固定、石蜡包埋头颈部鳞癌标本中人乳头瘤病毒状态的分类算法中

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